Western blot analysis of PTPRF in BeWo cells. Samples were incubated with PTPRF Polyclonal antibody (Product # PA5-110242) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Western blot analysis of PTPRF in MCF7, THP-1, mouse liver. Samples were incubated in polyclonal PTPRF antibody (Product # PA5-110242) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supplier Page from Thermo Fisher Scientific for PTPRF Antibody